Randall, on loan from Arsenal, was booked into a hotel the night before the game, but instead opted to return home in Milton Keynes and, after his car broke down on the M6 en route to the north west, he did not make the Edgeley Park clash.
Moore, who has yet to see Randall following the incident, has warned the 21-year-old to expect a verbal lashing and a fine.
"It is a lack of professionalism, he wouldn't have done it at Arsenal and it just shows disrespect for Rotherham United and we won't have it," Moore told Press Association Sport.
"It will be dealt with in house and he'll be fined but I want him to stay. He's a quality player, but he can't be seen to getting away with that. He wouldn't do it to Arsene Wenger and he won't do it to Ronnie Moore."
Randall's stay with the Millers has been blighted by injury and he has made just three appearances since joining in October. His deal is set to expire on January 25.